人Anti-Varicella-Zoster virus IgM ELISA试剂盒(VZV) (ab108783)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
人Anti-Varicella-Zoster virus IgM ELISA试剂盒(VZV) -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos. Serum 8 5.2% 批次间 样品 n Mean SD CV% Pos. Serum 16 4.2% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Varicella-Zoster virus (VZV) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Varicella-Zoster virus in Human serum and plasma.
A 96-well plate has been precoated with Varicella-Zoster virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Varicella-Zoster virus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Varicella-Zoster virus IgM sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgM Sample Diluent Colored green; White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Varicella-Zoster virus (IgM) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Varicella-Zoster virus (VZV) IgM Cut-off Control Colored yellow; Green cap 1 x 3ml Varicella-Zoster virus (VZV) IgM Negative Control Colored yellow; Blue cap 1 x 2ml Varicella-Zoster virus (VZV) IgM Positive Control Colored yellow; Red cap 1 x 2ml Varicella-Zoster virus anti-IgM HRP Conjugate Colored red; Black cap 1 x 20ml -
研究领域
相关产品
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Related Products
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108783 被引用在 1 文献中.
- Kombe Kombe AJ et al. Detection of Circulating VZV-Glycoprotein E-Specific Antibodies by Chemiluminescent Immunoassay (CLIA) for Varicella-Zoster Diagnosis. Pathogens 11:N/A (2022). PubMed: 35056014