概述

  • 产品名称人VAP-1 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3%
    批次间
    样品 n Mean SD CV%
    Overall 8 4.5%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    19 pg/ml
  • 范围
    31.3 pg/ml - 2000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 96 82% - 117%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s VAP-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VAP-1 in cell culture supernatant, serum, and plasma (EDTA, heparin) and amniotic fluid.

    VAP-1 specific antibodies have been precoated onto 96-well plates. Standards and samples are added to the wells and VAP-1 present in a sample is bound to the wells by the immobilized antibody. After incubation the wells are washed and biotinylated anti-Human VAP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is added to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VAP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • 组织特异性Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
  • 序列相似性Belongs to the copper/topaquinone oxidase family.
  • 翻译后修饰Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • Amine oxidase copper containing 3
    • AOC3
    • AOC3_HUMAN
    • Copper amine oxidase
    • HPAO
    • Membrane primary amine oxidase
    • Semicarbazide-sensitive amine oxidase
    • SSAO
    • VAP-1
    • VAP1
    • Vascular adhesion protein 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human VAP-1 ELISA Kit 图像

  • Representative Standard Curve using ab119564.

实验方案

Human VAP-1 ELISA Kit (ab119564)参考文献

ab119564 has not yet been referenced specifically in any publications.

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