概述

  • 产品名称
    人VAP-1 ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3%
    批次间
    样品 n Mean SD CV%
    Overall 8 4.5%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    19 pg/ml
  • 范围
    31.3 pg/ml - 2000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Serum 96 82% - 117%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s VAP-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VAP-1 in cell culture supernatant, serum, and plasma (EDTA, heparin) and amniotic fluid.

    VAP-1 specific antibodies have been precoated onto 96-well plates. Standards and samples are added to the wells and VAP-1 present in a sample is bound to the wells by the immobilized antibody. After incubation the wells are washed and biotinylated anti-Human VAP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is added to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VAP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119564.

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

There are currently no Customer reviews or Questions for ab119564.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册