The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-USP6 antibody (ab4553)
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Deubiquitinating enzyme 6
Ubiquitin carboxyl-terminal hydrolase 6
Ubiquitin specific protease 6
Ubiquitin thiolesterase 6
Ubiquitin-specific-processing protease 6
功能Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
组织特异性Testis specific. Expressed in various cancer cell lines.
疾病相关Note=A chromosomal aberration involving USP6 is a common genetic feature of aneurysmal bone cyst, a benign osseous neoplasm. Translocation t(16;17)(q22;p13) with CDH11. The translocation generates a fusion gene in which the strong CDH11 promoter is fused to the entire USP6 coding sequence, resulting in USP6 transcriptional up-regulation.
序列相似性Belongs to the peptidase C19 family. Contains 1 Rab-GAP TBC domain.
结构域The Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6.
翻译后修饰Monubiquitinated; ubiquitination is calmodulin and calcium dependent.
细胞定位Cell membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.