人Urokinase type Plasminogen Activator ELISA试剂盒(ab108917)

概述

  • 产品名称人Urokinase type Plasminogen Activator ELISA试剂盒
    参阅全部 Urokinase type Plasminogen Activator 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Tissue
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.2 ng/ml
  • 范围
    0.25 ng/ml - 8 ng/ml
  • 复检

    98.5 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产���概述

    Abcam’s Urokinase type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Urokinase type Plasminogen Activator (uPA) in plasma, serum, urine, milk, cell culture supernatants and tissue extracts.

     

    A Urokinase type Plasminogen Activator specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Urokinase type Plasminogen Activator specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Urokinase type Plasminogen Activator captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Specifically cleave the zymogen plasminogen to form the active enzyme plasmin.
  • 组织特异性Expressed in the prostate gland and prostate cancers.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰Phosphorylation of Ser-158 and Ser-323 abolishes proadhesive ability but does not interfere with receptor binding.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • ATF
    • BDPLT5
    • Plasminogen activator urinary
    • PLAU
    • QPD
    • u PA
    • U-plasminogen activator
    • uPA
    • URK
    • UROK_HUMAN
    • Urokinase-type plasminogen activator chain B
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Urokinase type Plasminogen Activator ELISA Kit 图像

  • Representative Standard Curve using ab108917.

实验方案

Human Urokinase type Plasminogen Activator ELISA Kit (ab108917)参考文献

ab108917 has not yet been referenced specifically in any publications.

Product Wall



Both ab108914 and ab108917 contain 12, 8 well strips.


You are correct that you should be able to detect tPA and uPA from cell culture supernatants or cell homogenates with both these kits.

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.
Read More

Thank you for your email.

We generally recommend our customer using 400-500ul of lysis buffer per 5-10 mg of tissues however you may have to adjust it based upon your results.

500ul lysis buffer for 1 million cells would be ideal.
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"