人uPAR ELISA试剂盒(URKR) (ab119612)

概述

  • 产品名称人uPAR ELISA试剂盒(URKR)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 4 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human uPAR (URKR) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human uPAR (URKR) in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

    A uPAR (URKR) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for uPAR (URKR) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human uPA Receptor amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.
  • 组织特异性Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.
  • 序列相似性Contains 3 UPAR/Ly6 domains.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 别名
    • CD87
    • Monocyte activation antigen Mo3
    • PLAUR
    • U-PAR
    • uPAR
    • UPAR_HUMAN
    • Urokinase plasminogen activator surface receptor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human uPAR ELISA Kit (URKR) 图像

  • Representative standard curve using ab119612

实验方案

Human uPAR ELISA Kit (URKR) (ab119612)参考文献

ab119612 has not yet been referenced specifically in any publications.

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