人Triiodothyronine ELISA试剂盒(T3) (ab108663)

概述

  • 产品名称人Triiodothyronine ELISA试剂盒(T3)
    参阅全部 Triiodothyronine (T3) 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Low 20 1.37pg/ml 0.16 11.9%
    Normal 20 4.21pg/ml 0.17 4.1%
    High 20 7.1pg/ml 0.17 2.4%
    批次间
    样品 n Mean SD CV%
    Low 10 1.4pg/ml 0.15 10.7%
    Normal 10 4.4pg/ml 0.23 5.2%
    High 10 7pg/ml 0.3 4.2%
  • 测试
    1 x 96 test
  • 样品类型
    Serum
  • 检测类型Competitive
  • 范围
    0.9 pg/ml - 19 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Triiodothyronine (T3) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Triiodothyronine (T3) in Human serum.

    A 96-well plate has been pre-coated with Anti-Triiodothyronine antibodies. Samples, standards and the Triiodothyronine HRP Conjugate are added to the wells, where Triiodothyronine in the sample competes with the added Triiodothyronine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Triiodothyronine in the sample and the intensity is measured at 450 nm.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab108663 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Competitive ELISA Use at an assay dependent dilution.

    Human Triiodothyronine ELISA Kit (T3) 图像

    • Representative standard curve using ab108663

    实验方案

    Human Triiodothyronine ELISA Kit (T3) (ab108663)参考文献

    ab108663 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    This ELISA kit was used with cell supernatant and the cell extract of HEK293 after homogenisation. The cells were transfected with Type-II Deiodinase variants and were later treated with a T4 medium at a concentration of 20pM. The kit was successful in measuring the concentration of T3 in both the supernatant and cell extract yielding a range of results from 2.9-8.6pg/mL.
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    Verified customer

    提交于 Jan 06 2016

    Abreviews
    This kit is not sensitive enough to detect any differences in free T3 generated by cells in cell culture supernatant. It can easily detect exogenous T3 added at 20 nM with strong signal.
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    Verified customer

    提交于 Oct 30 2015

    application to mouse

    Good Good 4/5 (Ease of Use)
    Abreviews
    It seems that mouse serum can be used with this kit, yielding values in the range of 2-6 pg/mL, which is in the range of previously reported values for human free T3 (http://www.globalrph.com/labs_t.htm#T4_(thyroxine)_Total_) and mouse free T3 (http://genesdev.cshlp.org/content/13/10/1329.full - see figure 2A, WT).
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    Abcam user community

    Verified customer

    提交于 Sep 28 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"