人Anti-Trichinella spiralis IgG ELISA试剂盒(ab108780)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
概述
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产品名称
人Anti-Trichinella spiralis IgG ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Pos. Serum 8 3.9% 批次间 样品 n Mean SD CV% Pos. Serum 4 5.2% -
样品类型
Serum, Hep Plasma, Cit plasma -
检测类型
Indirect -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s anti-Trichinella spiralis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma.
A 96-well plate has been precoated with Trichinella spiralis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Trichinella spiralis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Trichinella spiralis IgG sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Colored yellow; White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Trichinella spiralis (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Trichinella spiralis IgG Cut-off Control Colored yellow; Green cap 1 x 3ml Trichinella spiralis IgG Negative Control Colored yellow; Blue cap 1 x 2ml Trichinella spiralis IgG Positive Control Colored yellow; Red cap 1 x 2ml Trichinella spiralis Protein A HRP Conjugate Colored blue; Black cap 1 x 20ml -
研究领域
图片
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab108780 被引用在 1 文献中.
- Liu Y et al. Recombinant cystatin-like protein-based competition ELISA for Trichinella spiralis antibody test in multihost sera. PLoS Negl Trop Dis 15:e0009723 (2021). PubMed: 34432796