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Abcam’s anti-Treponema pallidum IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Treponema pallidum in Human serum and plasma.
A 96-well plate has been precoated with Treponema pallidum antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Treponema pallidum-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Treponema pallidum IgG sample captured in plate.
|组件||标识符||1 x 96 tests|
|20X Washing Solution||White cap||1 x 50ml|
|Cover foil||1 unit|
|IgG Sample Diluent||Colored yellow; White cap||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||Yellow cap||1 x 15ml|
|Treponema pallidum (IgG) Coated Microplate (12 x 8 wells)||12 strips of 8 wells||1 unit|
|Treponema pallidum anti-IgG HRP Conjugate||Colored blue; Black cap||1 x 20ml|
|Treponema pallidum Cut-off Control||Colored yellow; Green cap||1 x 3ml|
|Treponema pallidum Negative Control||Colored yellow; Blue cap||1 x 2ml|
|Treponema pallidum Positive Control||Colored yellow; Red cap||1 x 2ml|
Our Abpromise guarantee covers the use of ab108779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent dilution.|
ab108779 has not yet been referenced specifically in any publications.