人Anti-Treponema pallidum IgG ELISA试剂盒(ab108779)

概述

  • 产品名称人Anti-Treponema pallidum IgG ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Pos. Serum 8 1.5%
    批次间
    样品 n Mean SD CV%
    Pos. Serum 4 5.1%
  • 测试
    1 x 96 test
  • 样品类型
    Serum, Plasma
  • 检测类型Indirect
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s anti-Treponema pallidum IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Treponema pallidum in Human serum and plasma.

     

    A 96-well plate has been precoated with Treponema pallidum antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Treponema pallidum-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Treponema pallidum IgG sample captured in plate.

  • 经测试应用适用于: Indirect ELISAmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab108779 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Indirect ELISA Use at an assay dependent dilution.

    实验方案

    Human Anti-Treponema pallidum IgG ELISA Kit (ab108779)参考文献

    ab108779 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam.

    I apologize for the delay in responding. The plates are coated with recombinant r15, r17, and r44,5 protein.

    Please let me know if I can be of any further assistance.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"