人TREM1 ELISA试剂盒(ab100659)

概述

  • 产品名称人TREM1 ELISA试剂盒
    参阅全部 TREM1 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 70 pg/ml
  • 范围
    0.069 ng/ml - 50 ng/ml
  • 复检

    > 100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 124.4 106% - 134%
    Serum 125.5 91% - 145%
    Plasma 116 94% - 115%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TREM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM1 in serum, plasma and cell culture supernatants. (Human TREM1 concentration is pretty low in normal serum, it may not be detected in this assay).

    This assay employs an antibody specific for Human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性TREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock.
  • 细胞定位Secreted and Cell membrane.
  • 别名
    • CD354
    • TREM 1
    • Triggering receptor expressed on monocytes 1
    • Triggering receptor expressed on myeloid cells 1
    • Triggering receptor TREM 1
    • Triggering receptor TREM1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TREM1 ELISA Kit 图像

  • Representative standard curve using ab100659

  • Representative standard curve using ab100659

实验方案

Human TREM1 ELISA Kit (ab100659)参考文献

ab100659 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

I can confirm that you will find all of the available QC data about serum and plasma near the end of the protocol booklet including spiking&recovery and linearity tests.

Furthermore I like to confirm tha...

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Thank you for contacting us.

The kit ab100659 TREM1 Human ELISA Kit is suitable to quantify the soluble form of human TREM-1.

Unfortunately, the concentrations used to coat the microplate and the one for the biotynilated antibody ar...

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The report I received from the laboratory tells us that it will be impossible to predict if this ELISA kit can detect the splice variant you are interested in, given the information we have about the antibodies.

From the UniProt page for hum...

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Thank you for your inquiry.

It is recommended to plot the standard curve using a log-log model as stated in the Protocol Booklet. Log-log (common log) is recommended as this model fits most data sets well. However, other graphing models can ...

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Thank you for contacting us and your interest in our products.
The TREM1 Human ELISA Kit (ab100659) uses the diluent A and B in sample preparation of serum/plasma and cell culture medium/urine respectively.
The Assay Diluent A is optimized fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"