概述

  • 产品名称人Transferrin ELISA试剂盒
    参阅全部 Transferrin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.6%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    = 0.09 µg/ml
  • 范围
    0.098 µg/ml - 25 µg/ml
  • 复检

    99 %

  • 检测时间
    2h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Transferrin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in plasma and serum samples.

    A Transferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Transferrin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Transferrin captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Defects in TF are the cause of atransferrinemia (ATRAF) [MIM:209300]. Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia.
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Apotransferrin
    • Beta 1 metal binding globulin
    • Beta-1 metal-binding globulin
    • DKFZp781D0156
    • PRO1400
    • PRO1557
    • PRO2086
    • Serotransferrin
    • Serotransferrin precursor
    • Siderophilin
    • TF
    • TFQTL1
    • Transferin
    • Transferrin
    • TRFE_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human Transferrin ELISA Kit 图像

  • Representative Standard Curve using ab108911.

实验方案

Human Transferrin ELISA Kit (ab108911)参考文献

This product has been referenced in:
  • Gomes JA  et al. Brain iron metabolism and brain injury following subarachnoid hemorrhage: iCeFISH-pilot (CSF iron in SAH). Neurocrit Care 21:285-93 (2014). ELISA ; Human . Read more (PubMed: 24710655) »
  • Yu SY  et al. Sleep disorders in Parkinson's disease: clinical features, iron metabolism and related mechanism. PLoS One 8:e82924 (2013). Sandwich ELISA ; Human . Read more (PubMed: 24376607) »

See all 2 Publications for this product

Product Wall



The transferring kit contains two antibodies against full length recombinant protein and purified proteins. Human IgG3 kit contains two antibodies against full length purified proteins.

I am afraid we do not map the epitopes of thes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"