人Transferrin ELISA试剂盒(ab108902)

概述

  • 产品名称人Transferrin ELISA试剂盒
    参阅全部 Transferrin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 9.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 1.5 ng/ml
  • 范围
    1.563 ng/ml - 100 ng/ml
  • 复检

    96.5 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.


    A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
  • 组织特异性Expressed by the liver and secreted in plasma.
  • 疾病相关Atransferrinemia
  • 序列相似性Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • ATRAF
    • Atransferrinemia
    • Beta-1 metal-binding globulin
    • PRO1400
    • Serotransferrin
    • Siderophilin
    • TF
    • Transferrin
    • TRFE_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Transferrin ELISA Kit 图像

  • Representative Standard Curve using ab108902.

实验方案

Human Transferrin ELISA Kit (ab108902)参考文献

ab108902 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience in awaiting our reply in the matter of compaibility with the kit. It is difficulty to know whether this will work. If the conjugation block the antibody binding site, it will interfere the assay. If not, it will be fine. ...

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Abreview for Transferrin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.1ug standard µg
Type of Kit Used: ELISA
Positive control: Provided transferrin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human transferrin. Did not recognize bovine transferrin in FBS.
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Abcam user community

Verified customer

提交于 Jul 04 2012

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"