概述

  • 产品名称
    人Transferrin ELISA试剂盒
    参阅全部 Transferrin 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.7%
    批次间
    样品 n Mean SD CV%
    Overall 9.9%
  • 样品类型
    Cell culture supernatant, Saliva, Milk
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 1 ng/ml
  • 范围
    1.563 ng/ml - 100 ng/ml
  • 复检

    97 %

  • 检测时间
    4h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Tranferrin Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Transferrin in urine, milk saliva, CSF and cell culture supernatants.


    A Tranferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tranferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tranferrin captured in plate.


    Get results in 90 minutes with Human Transferrin ELISA Kit (ab187391) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

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应用

Our Abpromise guarantee covers the use of ab108902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab108902.

实验方案

文献

This product has been referenced in:
  • Mehta SR  et al. Cerebrospinal fluid cell-free mitochondrial DNA is associated with HIV replication, iron transport, and mild HIV-associated neurocognitive impairment. J Neuroinflammation 14:72 (2017). ELISA ; Human . Read more (PubMed: 28359324) »
  • Patton SM  et al. Cerebrospinal fluid (CSF) biomarkers of iron status are associated with CSF viral load, antiretroviral therapy, and demographic factors in HIV-infected adults. Fluids Barriers CNS 14:11 (2017). ELISA ; Human . Read more (PubMed: 28427421) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your patience in awaiting our reply in the matter of compaibility with the kit. It is difficulty to know whether this will work. If the conjugation block the antibody binding site, it will interfere the assay. If not, it will be fine. ...

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Abreview for Transferrin Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2)
Specification: HepG2
Amount of Sample: 0.1ug standard µg
Type of Kit Used: ELISA
Positive control: Provided transferrin standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human transferrin. Did not recognize bovine transferrin in FBS.
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Abcam user community

Verified customer

提交于 Jul 04 2012

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially opti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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