概述

  • 产品名称人TRAIL ELISA试剂盒(CD253)
    参阅全部 TRAIL 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    A 40 1500pg/ml 82.9 5.5%
    B 33 116.3pg/ml 8.7 7.4%
    批次间
    样品 n Mean SD CV%
    A 12 1619pg/ml 62.8 3.8%
    B 12 162.8pg/ml 13.1 8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 64 pg/ml
  • 范围
    93.75 pg/ml - 3000 pg/ml
  • 检测时间
    3h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TRAIL (CD253) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TRAIL in cell culture supernatants, buffered solutions or Human serum, plasma, and other body fluids.

    A monoclonal antibody specific for TRAIL has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TRAIL concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TRAIL are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TRAIL present in the samples.

    This kit will recognize both endogenous and recombinant Human TRAIL.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.
  • 细胞定位Cell Membrane; single-pass type II membrane protein.
  • 别名
    • Apo 2 ligand
    • APO2L
    • CD253
    • Protein TRAIL
    • TL2
    • TNF related apoptosis inducing ligand
    • Tumor necrosis factor ligand superfamily member 10
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TRAIL ELISA Kit (CD253) 图像

  • TRAIL measured in undiluted cell culture supernatants from control (-) or stimulated ((P/I); 48 hours with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116)) Jurkat cells (duplicates +/- SD).

  • TRAIL measured in undiluted biological fluids (duplicates +/- SD).

  • Representative Standard Curve using ab46074

实验方案

Human TRAIL ELISA Kit (CD253) (ab46074)参考文献

This product has been referenced in:
  • Greenwood EJ  et al. Simian Immunodeficiency Virus Infection of Chimpanzees (Pan troglodytes) Shares Features of Both Pathogenic and Non-pathogenic Lentiviral Infections. PLoS Pathog 11:e1005146 (2015). Sandwich ELISA . Read more (PubMed: 26360709) »

See 1 Publication for this product

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Thank you for your call today and for letting us know about the trouble with ab46074.
As we discussed, I'm sending a free of charge replacement kit, which should arrive tomorrow on the order ***.
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At this time we haveno information regarding the cross reactivity between our human TRAIL ELISA (ab46074)and the murine protein.

Therefore, I can offer a discount off a future purchase if you buy ab46074 now, test it inmouse and submit feedba...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"