Abcam’s TNFRSF18 (GITR) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TNFRSF18 in cell lysates and tissue lysates.
This assay employs an antibody specific for Human TNFRSF18 coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFRSF18 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TNFRSF18 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF18 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells.
Activation inducible TNFR family receptor
Glucocorticoid induced TNFR related protein
Tumor necrosis factor receptor superfamily member 18