概述

  • 产品名称人TNF beta ELISA试剂盒
    参阅全部 TNF beta 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3 8%
    批次间
    样品 n Mean SD CV%
    Overall 3 10.2%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    4.6 pg/ml
  • 范围
    15.6 pg/ml - 1000 pg/ml
  • 复检

    105 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 105 87% - 130%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TNF beta Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TNF beta concentrations in cell culture supernatant, serum and plasma.

    TNF beta specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with HRP-conjugated TNF beta detection antibody and the microplate is then incubated at room temperature and unbound conjugates are then washed away using wash buffer. TMB is the added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the TNF beta amount of sample captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • 疾病相关Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 细胞定位Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
  • Information by UniProt
  • 别名
    • LTalpha
    • DIF
    • LT
    • LT alpha
    • LT-alpha
    • Lta
    • Lymphotoxin alpha
    • Lymphotoxin alpha (TNF superfamily, member 1)
    • Lymphotoxin-alpha
    • TNF B
    • TNF superfamily member 1
    • TNF, lymphocyte-derived
    • TNF-beta
    • TNFB
    • TNFB_HUMAN
    • TNFbeta
    • TNFSF1
    • TNLG1E
    • Tumor necrosis factor beta
    • tumor necrosis factor ligand 1E
    • Tumor necrosis factor ligand superfamily member 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TNF beta ELISA Kit 图像

  • Representative Standard Curve using ab119561.

实验方案

Human TNF beta ELISA Kit (ab119561)参考文献

ab119561 has not yet been referenced specifically in any publications.

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