人TNF alpha酶联免疫吸附(ELISA)套装(without plates) (ab47347)

概述

  • 产品名称
    人TNF alpha酶联免疫吸附(ELISA)套装(without plates)
    参阅全部 TNF alpha 试剂盒
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 10 pg/ml
  • 范围
    25 pg/ml - 800 pg/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TNFα Human in vitro ELISA Set is designed for the quantitative measurement of TNFα in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    The TNF alpha Elipair includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    This Elipair set is intended to measure human TNF alpha in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

     

    Recognizes both natural and recombinant human TNF alpha.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Reagents

性能

应用

Our Abpromise guarantee covers the use of ab47347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 图片

    • TNF alpha Standard curve ranging from 25 to 800 pg/mL

    文献

    ab47347 has not yet been referenced specifically in any publications.

    客户评价及客户问答


    The non-biotinylated version of the detection antibody is available on our catalog as ab27381.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    The detection antibody is available as ab27381.

    Thank you for your inquiry.
    HRP-Streptavidin is provided at 1.0mg/ml for a final working concentration of 0.15µg/ml when diluted according to the recommendations.
    I hope this information helps. Please contact us with any other questions.

    Thank you for contacting us.

    We do not sell individual components for ab47347 at the moment.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Us...

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    Thank you very much for your reply.

    I am glad that you have found some kits that will be suitable for your assay. If you need any help standardizing your methods, please let me know and I'll be happy to assist.

    Have a wonderful day.

    Thank you very much for contacting us with your questions, and for your patience while I have been in touch with the lab regarding your enquiry.

    For the TAC kit ab65329, you can use a general lysis buffer such as RIPA to prepare the lysates.T...

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    For ab47347, we provide capture antibody at 1.0mg/ml and detection at 50µg/ml (25µg/vial).
    I hope this is helpful. Please contact me again if you have any further questions.

    Thanks for your enquiry. Please see below for answers to your questions:

    Response to question 1: No, 24 hr drying time is not necessary. After tapping the plate to remove excess liquid from the wells, it should be totally dry much faster than...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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