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Abcam’s Tissue Type Plasminogen Activator (TPA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Type Plasminogen Activator in plasma, serum, urine, saliva, milk, cell culture supernatants and tissue extracts.
A TPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TPA captured in plate.
|组件||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human TPA Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
|TPA Microplate (12 x 8 well strips)||1 unit|
|TPA Standard||1 vial|
Our Abpromise guarantee covers the use of ab108914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 2-10 fold.
Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Human samples diluted 3-27 fold. Rat and mouse samples diluted 1-10 fold.
Representative Standard Curve Using ab108914.
ab108914 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"