人组织Type Plasminogen Activator ELISA试剂盒(TPA) (ab108914)

概述

  • 产品名称人组织Type Plasminogen Activator ELISA试剂盒(TPA)
    参阅全部 Tissue type Plasminogen Activator 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.1%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.03 ng/ml
  • 范围
    0.031 ng/ml - 2 ng/ml
  • 复检

    98 %

  • 检测时间
    4h 0m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Tissue Type Plasminogen Activator (TPA) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Type Plasminogen Activator in plasma, serum, urine, saliva, milk, cell culture supernatants and tissue extracts.


    A TPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TPA captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 组织特异性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 疾病相关Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 结构域Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻译后修饰The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 别名
    • Alteplase
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t-PA
    • T-plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Tissue Type Plasminogen Activator ELISA Kit (TPA) 图像

  • Tissue Type Plasminogen Activator measured in biological fluids showing quantity (ng) per mL of tested sample. Samples diluted 2-10 fold.

  • Tissue Type Plasminogen Activator  measured in biological fluids showing quantity (ng) per mL of tested sample. Human samples diluted 3-27 fold. Rat and mouse samples diluted 1-10 fold.

  • Representative Standard Curve Using ab108914.

实验方案

Human Tissue Type Plasminogen Activator ELISA Kit (TPA) (ab108914)参考文献

ab108914 has not yet been referenced specifically in any publications.

Product Wall



Both ab108914 and ab108917 contain 12, 8 well strips.


You are correct that you should be able to detect tPA and uPA from cell culture supernatants or cell homogenates with both these kits.

Thank you for contacting us.

I can confirm that it should read the samplescan be stored at -20 degrees Celsius or lower. I apologize for this error and we will correct it.

I hope this information is helpful to you. Please do not h...

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