人组织type Plasminogen Activator ELISA试剂盒(ab119563)

概述

  • 产品名称人组织type Plasminogen Activator ELISA试剂盒
    参阅全部 Tissue type Plasminogen Activator 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3.6%
    批次间
    样品 n Mean SD CV%
    Overall 8 6.5%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    6 pg/ml
  • 范围
    15.6 pg/ml - 1000 pg/ml
  • 复检

    特定样本复检结果
    样品类型 平均% 范围
    Serum 98 77% - 109%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Tissue type Plasminogen Activator Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Tissue type Plasminogen Activator concentrations in Cell culture supernatant and serum.


    Tissue type Plasminogen Activator specific antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells along with an HRP-conjugated Tissue type Plasminogen Activator detection antibody and the microplate is then incubated at room temperature and unbound proteins are then washed away with a wash buffer. TMB is then added and catalyzed by HRP to produce a blue color product that subsequently changes to yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the Tissue type Plasminogen Activator amount of sample captured on the plate.


    For higher sensitivity in only 90 minutes, we recommend Human Tissue Plasminogen Activator ELISA Kit (ab190812) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 存放说明Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 2 units
    HRP-Conjugate anti-human Tissue type Plasminogen Activator polyclonal antibody 1 x 70µl
    Human Tissue type Plasminogen Activator Standard lyophilized 2 vials
    Microplate coated with polyclonal antibody to Human Tissue type Plasminogen Activator (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • 研究领域
  • 功能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 组织特异性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 疾病相关Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 结构域Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻译后修饰The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt
  • 别名
    • Alteplase
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t-PA
    • T-plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Tissue type Plasminogen Activator ELISA Kit 图像

  • Representative Standard Curve using ab119563.

实验方案

Human Tissue type Plasminogen Activator ELISA Kit (ab119563)参考文献

ab119563 has not yet been referenced specifically in any publications.

Product Wall

testing rhesus crossreactivity

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The Factor tPA ELISA, using the Abcam human ELISA kit ab119563, lt GR257353-2, was performed by an experienced laboratory technician at an accredited university. The goal of the experiment was to analyze the linearity of dilutions of rhesus macaque EDTA-prepared plasma samples and serum.

Overall, the kit, like other Abcam ELISA kits, was easy to follow, clear in terms of directions, and brief yet thorough. The laboratory technician had no problems understanding any of the protocol’s steps.

Our experiment consisted of using EDTA-prepared Rhesus Macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma and Serum samples were diluted 1:5, 1:10, 1:20, and 1:40 in the given diluent as duplicates. The rest of the assay was performed per protocol’s instructions.

Absorbance measurements of the diluted samples were very comparable between duplicates. Samples diluted at 1:5, 1:10, and 1:20 gave similar extrapolated results and seemed to be in a linear range.

We performed a spike recovery as follows: 30uL of 2000ng/mL protein (from resuspension of the std) was spiked into 90ul of diluent, plasma, or serum. In Diluent, the recovery was 110% at 1:5, 1:10, and 1:20. In Plasma, recovery was 70% at the 1:20 dilution, but not detectable at 1:10 or 1:5. In the serum, recovery ranged between 25% and 40% in the tested dilutions.

Overall, we would recommend this kit for its ease of use and positive crossreactivity with rhesus samples.
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提交于 Jun 01 2016

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