人组织Factor Pathway抑制剂ELISA试剂盒(TFPI) (ab108904)
Key features and details
- Sensitivity: 0.081 ng/ml
- Range: 0.625 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人组织Factor Pathway抑制剂ELISA试剂盒(TFPI)
参阅全部 TFPI 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 5% 批次间 样品 n Mean SD CV% Overall 9.9% -
样品类型
Cell culture supernatant, Milk, Serum, Plasma, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
> 0.081 ng/ml -
范围
0.625 ng/ml - 5 ng/ml -
回收率
98 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.
A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.
Get results in 90 minutes with Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Tissue Factor Pathway Inhibitor Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Sealing Tapes 3 units Stop Solution 1 x 12ml Tissue Factor Pathway Inhibitor Microplate (12 x 8 well strips) 1 x 96 units Tissue Factor Pathway Inhibitor Standard 2 vials -
研究领域
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功能
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
组织特异性
Mostly in endothelial cells. -
序列相似性
Contains 3 BPTI/Kunitz inhibitor domains. -
结构域
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
翻译后修饰
O-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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别名
- Anti convertin
- EPI
- Extrinsic pathway inhibitor
see all -
数据库链接
- Entrez Gene: 7035 Human
- Omim: 152310 Human
- SwissProt: P10646 Human
- Unigene: 516578 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab108904 被引用在 6 文献中.
- Ma XP et al. Transthyretin increases migration and invasion of rat placental trophoblast cells. FEBS Open Bio 10:1568-1576 (2020). PubMed: 32533762
- Martínez González J et al. Bioequivalence of a biosimilar enoxaparin sodium to Clexane® after single 100 mg subcutaneous dose: results of a randomized, double-blind, crossover study in healthy volunteers. Drug Des Devel Ther 12:575-582 (2018). PubMed: 29593380
- Ansell JE et al. Single-dose ciraparantag safely and completely reverses anticoagulant effects of edoxaban. Thromb Haemost 117:238-245 (2017). PubMed: 27853809
- Vieira ML et al. Modulation of Hemostatic and Inflammatory Responses by Leptospira Spp. PLoS Negl Trop Dis 10:e0004713 (2016). PubMed: 27167223
- Abu El-Asrar AM et al. Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy. Mol Vis 22:424-35 (2016). PubMed: 27168718
- Park MY et al. Biomarkers of kidney injury and klotho in patients with atherosclerotic renovascular disease. Clin J Am Soc Nephrol 10:443-51 (2015). Sandwich ELISA ; Human . PubMed: 25542906