人组织Factor Pathway Inhibitor ELISA试剂盒(TFPI) (ab108904)

概述

  • 产品名称人组织Factor Pathway Inhibitor ELISA试剂盒(TFPI)
    参阅全部 Tissue Factor Pathway Inhibitor 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 7.4%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Milk, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    > 0.12 ng/ml
  • 范围
    0.313 ng/ml - 20 ng/ml
  • 复检

    96.5 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Tissue Factor Pathway Inhibitor (TFPI) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor Pathway Inhibitor concentrations in plasma, serum, milk, cerebrospinal fluid and cell culture samples.


    A Tissue Factor Pathway Inhibitor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor Pathway Inhibitor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor Pathway Inhibitor captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human Tissue Factor Pathway Inhibitor ELISA Kit (ab188392) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 组织特异性Mostly in endothelial cells.
  • 序列相似性Contains 3 BPTI/Kunitz inhibitor domains.
  • 结构域This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Secreted and Microsome membrane.
  • Information by UniProt
  • 别名
    • EPI
    • Extrinsic pathway inhibitor
    • LACI
    • Lipoprotein-associated coagulation inhibitor
    • TFPI
    • TFPI1_HUMAN
    • Tissue factor pathway inhibitor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) 图像

  • TFPI measured in various samples showing quantity (ng) per mL of tested sample

  • Representative Standard Curve using ab108904.

实验方案

Human Tissue Factor Pathway Inhibitor ELISA Kit (TFPI) (ab108904)参考文献

This product has been referenced in:
  • Park MY  et al. Biomarkers of kidney injury and klotho in patients with atherosclerotic renovascular disease. Clin J Am Soc Nephrol 10:443-51 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25542906) »

See 1 Publication for this product

Product Wall

We can confirm that heparin does not interfere with the assay. However, we are not sure about protamine. This has not been tested before.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"