人组织Factor ELISA试剂盒(CD142) (ab108903)

概述

  • 产品名称人组织Factor ELISA试剂盒(CD142)
    参阅全部 Tissue Factor 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7%
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma, Cell culture extracts, Tissue
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 20 pg/ml
  • 范围
    12.5 pg/ml - 400 pg/ml
  • 复检

    97.5 %

  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Tissue Factor (CD142) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Tissue Factor concentrations in plasma, serum, tissue extracts and cell culture supernatants.

    A Tissue Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Tissue Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tissue Factor captured in plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
  • 序列相似性Belongs to the tissue factor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD142
    • CD142 antigen
    • Coagulation factor III
    • Coagulation factor III (thromboplastin tissue factor)
    • F3
    • FLJ17960
    • TF
    • TF_HUMAN
    • TFA
    • Thromboplastin
    • Tissue factor
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Tissue Factor ELISA Kit (CD142) 图像

  • CD142 measured in cell lysates showing quantity (pg) per 1 million cells tested

  • CD142 measured in various biofluids showing quantity (pg) per mL of tested sample

  • Representative Standard Curve using ab108903.

实验方案

Human Tissue Factor ELISA Kit (CD142) (ab108903)参考文献

ab108903 has not yet been referenced specifically in any publications.

Product Wall

Testing crossreactivity in Rhesus EDTA plasma

Good Excellent 5/5 (Ease of Use)
Abreviews
The Tissue Factor ELISA, using the Abcam human ELISA kit, was performed by an experienced laboratory technician at an accredited university. The goal of the experiment was to analyze the linearity of dilutions of rhesus macaque EDTA-prepared plasma samples, but more specifically, to analyze the cross reactivity for rhesus macaque.

Overall, the kit, like other Abcam ELISA kits, was easy to follow, clear in terms of directions, and brief yet thorough. The laboratory technician had no problems understanding any of the protocol’s steps.

Our experiment consisted of using EDTA-prepared Rhesus Macaque plasma samples from N=3 M. mulatta. Samples were stored prior to thaw at -80 degrees Celsius. Plasma was diluted to several different dilutions, as well as the suggested dilution from the kit (1:2, 1:4, 1:8, and 1:16) in the given diluent. The rest of the assay was performed per the protocol’s instructions.

Absorbance measurements of the diluted samples were very comparable between duplicates however, there was a clear lack of linearity between all dilutions’ Tissue Factor value for each sample (see image).

The standard curve was clean with a good range of ODs (see image).

The technician also performed a spike recovery, and found that, between pure diluent with known standard and plasma with known standard, plasma masked the known standard’s concentration in detection using this assay. A spiked concentration of 50 pg/mL was added to pure plasma, then the sample was diluted the same as unspiked sample. Resultant values were the same as unspiked plasma, i.e. we could not detected the spiked protein in rhesus plasma.

Overall, we do not recommend this kit for detection of Tissue Factor in EDTA-prepared Rhesus macaque plasma samples.
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提交于 Jun 01 2016

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Please attach the results and send us as per your earliest convenience. It will be a great help if could send the protocol exactly as used in experiment.

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