人TIMP4 ELISA试剂盒(ab113328)

概述

  • 产品名称人TIMP4 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    28.67 pg/ml - 7000 pg/ml
  • 复检

    80 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.11 89% - 104%
    Serum 73.49 67% - 82%
    Plasma 72.83 67% - 81%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TIMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP4 in serum, plasma (collect plasma using EDTA and citrate as an anticoagulant. Heparin is not recommended), cell culture supernatants.

    This assay employs an antibody specific for Human TIMP4 coated on a 96- well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 别名
    • Metalloproteinase inhibitor 4
    • TIMP 4
    • TIMP metallopeptidase inhibitor 4
    • Tissue inhibitor of metalloproteinase 4
    • Tissue inhibitor of metalloproteinases 4
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab113328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TIMP4 ELISA Kit 图像

  • Representative Standard Curve using ab113328

  • Representative Standard Curve using ab113328

实验方案

Human TIMP4 ELISA Kit (ab113328)参考文献

ab113328 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry. Unofrtunately there is no straightforward way to categorize which kits can be used with heparin and which cannot. You need to look at the datasheet or contact us regarding the specific kit, as different manufacturers and rea...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"