人TIMP4 ELISA试剂盒(ab113328)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 28.67 pg/ml - 7000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人TIMP4 ELISA试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 25 pg/ml -
范围
28.67 pg/ml - 7000 pg/ml -
回收率
80 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 96.11 89% - 104% Serum 73.49 67% - 82% Plasma 72.83 67% - 81% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s TIMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP4 in serum, plasma (collect plasma using EDTA and citrate as an anticoagulant. Heparin is not recommended), cell culture supernatants.
This assay employs an antibody specific for Human TIMP4 coated on a 96- well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
The standard is affinity purified.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated anti-Human TIMP4 2 vials Recombinant Human TIMP4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TIMP4 Microplate (12 strips x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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别名
- Metalloproteinase inhibitor 4
- TIMP 4
- TIMP metallopeptidase inhibitor 4
see all -
数据库链接
- Entrez Gene: 7079 Human
- Omim: 601915 Human
- SwissProt: Q99727 Human
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab113328 被引用在 1 文献中.
- Capistrano da Silva E et al. Determining MMP-2 and MMP-9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer. Vet Ophthalmol 24:279-287 (2021). PubMed: 33834598