概述

  • 产品名称人TIE2 ELISA试剂盒
    参阅全部 TIE2 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Serum 6 7.1%
    批次间
    样品 n Mean SD CV%
    Serum 6 6.3%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    0.03 ng/ml
  • 范围
    0.16 ng/ml - 10 ng/ml
  • 复检

    87 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TIE2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TIE2 concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    TIE2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an biotinylated TIE2 detection antibody and the microplate is then incubated overnight at 4 ºC. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of TIE2 captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
  • 组织特异性Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • 疾病相关Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
    Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • Angiopoietin-1 receptor
    • CD202b
    • CD202b antigen
    • EC 2.7.10.1
    • hTIE2
    • p140 TEK
    • soluble TIE2 variant 1
    • soluble TIE2 variant 2
    • Tek
    • TEK tyrosine kinase, endothelial
    • TIE 2
    • TIE2_HUMAN
    • Tunica interna endothelial cell kinase
    • Tyrosine-protein kinase receptor TEK
    • Tyrosine-protein kinase receptor TIE 2
    • Tyrosine-protein kinase receptor TIE-2
    • venous malformations, multiple cutaneous and mucosal
    • VMCM
    • VMCM1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TIE2 ELISA Kit 图像

  • Representative Standard Curve using ab119559.

实验方案

Human TIE2 ELISA Kit (ab119559)参考文献

ab119559 has not yet been referenced specifically in any publications.

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