概述

  • 产品名称
    人TIE2 ELISA试剂盒
    参阅全部 TIE2 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 20 pg/ml
  • 范围
    20.58 pg/ml - 15000 pg/ml
  • 复检

    97 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 76.59 68% - 92%
    Serum 110.9 101% - 118%
    Plasma 104.2 93% - 118%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TIE2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE2 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human TIE2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TIE2 2 vials
    Recombinant Human TIE2 Standard 2 vials
    Stop Solution 1 x 8ml
    TIE2 Microplate (12 x 8 wells) 1 unit
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 功能
    This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
  • 组织特异性
    Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • 疾病相关
    Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
    Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 别名
    • Angiopoietin-1 receptor
    • CD202b
    • CD202b antigen
    • EC 2.7.10.1
    • hTIE2
    • p140 TEK
    • soluble TIE2 variant 1
    • soluble TIE2 variant 2
    • Tek
    • TEK tyrosine kinase, endothelial
    • TIE 2
    • TIE2_HUMAN
    • Tunica interna endothelial cell kinase
    • Tyrosine-protein kinase receptor TEK
    • Tyrosine-protein kinase receptor TIE 2
    • Tyrosine-protein kinase receptor TIE-2
    • venous malformations, multiple cutaneous and mucosal
    • VMCM
    • VMCM1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100650

  • Representative standard curve using ab100650

实验方案

文献

ab100650 has not yet been referenced specifically in any publications.

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