概述

  • 产品名称人TIE1 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.6 ng/ml
  • 范围
    0.68 ng/ml - 500 ng/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 105.9 93% - 115%
    Serum 97 86% - 107%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TIE1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE1 in serum and cell culture supernatants. Human TIE1 (TIE1 concentration is pretty low in normal serum, it may not be detected in this assay). Plasma is not recommended.

    This assay employs an antibody specific for Human TIE1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.
  • 别名
    • EC 2.7.10.1
    • JTK14
    • receptor tyrosine kinase
    • soluble TIE1 variant 1
    • soluble TIE1 variant 2
    • soluble TIE1 variant 3
    • soluble TIE1 variant 4
    • soluble TIE1 variant 5
    • TIE
    • tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
    • tyrosine kinase with immunoglobulin like and EGF like domains 1
    • tyrosine-protein kinase receptor Tie 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TIE1 ELISA Kit 图像

  • Representative standard curve using ab100649

  • Representative standard curve using ab100649

实验方案

Human TIE1 ELISA Kit (ab100649)参考文献

ab100649 has not yet been referenced specifically in any publications.

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