人Anti-Tick-borne encephalitis virus IgG ELISA试剂盒(TBE) (ab108773)
概述
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产品名称人Anti-Tick-borne encephalitis virus IgG ELISA试剂盒(TBE)
参阅全部 Tick-Borne Encephalitis (TBE/FSME) Virus IgG 试剂盒 -
检测方法Colorimetric
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精确度
批次内 样品 n Mean SD CV% Standard B 7 6.8% Standard C 8 5.7% Standard D 8 4.7% Standard E 8 4.2% 批次间 样品 n Mean SD CV% Standard B 14 7.35% Standard C 16 8.3% Standard D 15 7.8% Standard E 16 6.7% -
样品类型Serum, Plasma
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检测类型Indirect
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实验步骤Multiple steps standard assay
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种属反应性与反应: Human
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产品概述
Abcam’s anti-Tick-borne encephalitis (TBE) virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Tick-borne encephalitis virus in Human serum and plasma.
A 96-well plate has been precoated with Tick-borne encephalitis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Tick-borne encephalitis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Tick-borne encephalitis IgG sample captured in plate.
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经测试应用适用于: Indirect ELISAmore details
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平台Microplate
性能
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存放说明Store at +4°C. Please refer to protocols.
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组件 标识符 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution 1 x 15ml Strip holder 1 unit TBE virus (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit TBE virus anti-IgG HRP Conjugate Blue, Black cap 1 x 20ml TBE virus IgG Standard A – 0 U/mL Yellow Blue cap 1 x 2ml TBE virus IgG Standard B – 50 U/mL Yellow, Green Cap 1 x 2ml TBE virus IgG Standard C – 130 U/mL Yellow, Yellow cap 1 x 2ml TBE virus IgG Standard D – 200 U/mL Yellow, Red cap 1 x 2ml TBE virus IgG Standard E – 300 U/mL Yellow, White cap 1 x 2ml TMB Substrate Solution Yellow cap 1 x 15ml -
研究领域
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应用
Our Abpromise guarantee covers the use of ab108773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Indirect ELISA | Use at an assay dependent dilution. |
实验方案
文献
ab108773 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"