人Thyroxine ELISA试剂盒(T4) (ab108662)

概述

  • 产品名称
    人Thyroxine ELISA试剂盒(T4)
    参阅全部 Thyroxine (T4) 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Low 16 3pg/ml 0.03 9.8%
    Normal 16 14pg/ml 0.06 4.5%
    High 16 36pg/ml 0.22 6.2%
    批次间
    样品 n Mean SD CV%
    Low 10 34pg/ml 0.04 11.5%
    Normal 10 13.5pg/ml 0.07 3.7%
    HIgh 10 36.9pg/ml 0.25 4.2%
  • 样品类型
    Serum
  • 检测类型
    Competitive
  • 灵敏度
    0.5 ng/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    ab108664, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Free Thyroxine (T4) concentration in Human serum.

    Thyroxine (T4) or 3,5,3’,5’-tetraiodothyronine is the most commonly measured thyroid hormone for the diagnosis of thyroid function. Thyroxine (T4) has its primary infuence on protein synthesis and oxygen consumption in virtually all tissues but it is also important for growth, development, and sexual maturation. Thyroxine (T4) is synthesized by the thyroid gland and is secreted into the bloodstream. Here the Thyroxine (T4) becomes bound to serum proteins for transport to the cells. The major transport protein is Thyroxine Binding Globulin (TBG) which normally accounts for 80% of the bound Thyroxine (T4). Other thyroid hormone binding proteins are Thyroxine Binding Prealbumin and Albumin. Most of the serum Thyroxine (T4) is bound to these transport proteins leaving only about 0.03% free to exert its effect on cells. It is the free Thyroxine (T4) (fT4) that represents the metabolically active fraction; for this reason the measurement of fT4 concentration is considered to be an indicator of patient thyroid status. Primary hypothyroidism results in underproduction of Thyroxine (T4) by the thyroid gland and consequently an abnormally low circulating fT4 concentration in the blood. Primary hyperthyroidism leads to excessive thyroid production on Thyroxine (T4) and resulting elevated fT4 concentration.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

图片

  • Standard Curve

实验方案

文献

ab108662 has not yet been referenced specifically in any publications.

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