人Thyroxine ELISA试剂盒(Free T4) (ab108686)

概述

  • 产品名称人Thyroxine ELISA试剂盒(Free T4)
    参阅全部 Thyroxine (T4) 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    0.5 pg/ml
  • 复检

    97.8 %

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroxine in Human serum and plasma.

     

    A 96-well plate has been precoated with anti-Thyroxine antibodies. Samples and the Thyroxine-HRP conjugate are added to the wells, where Thyroxine in the sample competes with the added
    Thyroxine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine in the sample and the intensity is measured at 450 nm.

     

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent dilution.

Human Thyroxine ELISA Kit (Free T4) 图像

  • Representative Standrad Curve using ab108686.

实验方案

Human Thyroxine ELISA Kit (Free T4) (ab108686)参考文献

ab108686 has not yet been referenced specifically in any publications.

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