人thyroid组织裂解液(adenocystic carcinoma) - insoluble fraction (male, 67 years) (ab44935)

概述

  • 产品名称人thyroid组织裂解液(adenocystic carcinoma) - insoluble fraction (male, 67 years)
    参阅全部 Thyroid 细胞裂解液
  • 描述
    人thyroid insoluble fraction
  • 常规说明

    Location: Thyroid. Diagnosis: Adenocystic carcinoma. Stage: IIB Grade: 2 Gross findings: Tumor size 3 cm. Cut section firm and hemorrhagic, well circumscribed. Microscopic findings: Tumor is characterized by proliferation of epithelial cells arranged in groups and separated by thin fibrosis with basophilic, irregular nuclei. Nuclear chromatine is coarse with prominent nucleoli. Tumor cells invade the stroma which is infiltrated by a small number of mononuclear inflammatory cells. The lymph nodes show metastasis (6/9).

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44935: Extraction 1: soluble protein fraction ab44932 Human thyroid tumor tissue lysate ab44930 Human thyroid normal tissue lysate (matched) Extraction 2: insoluble protein fraction ab44935 Human thyroid tumor tissue lysate ab44934 Human thyroid normal tissue lysate (matched)

性能

  • 形式Liquid
  • 存放说明Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 纯化说明Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究领域

    Human thyroid tissue lysate (adenocystic carcinoma) - insoluble fraction (male, 67 years) (ab44935)参考文献

    ab44935 has not yet been referenced specifically in any publications.

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