人Thyroid Stimulating Hormone ELISA试剂盒(TSH) (ab108659)

概述

  • 产品名称
    人Thyroid Stimulating Hormone ELISA试剂盒(TSH)
  • 检测方法
    Colorimetric
  • 样品类型
    Serum
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    = 0.05 U/ml
  • 范围
    0.05 µlU/ml - 10 µlU/ml
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Thyroid Stimulating Hormone in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroid Stimulating Hormone in Human serum.

    A 96-well plate has been precoated with anti-Thyroid Stimulating Hormone antibodies. Samples and standards and are added to the wells, where Thyroid Stimulating Hormone in the sample and standards binds to the precoated antibody. After incubation and washing, added Anti-Thyroid Stimulating Hormone HRP conjugate binds to the antibody-Thyroid Stimulating Hormone complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Thyroid Stimulating Hormone in the sample and the intensity is measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab108659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab108659

实验方案

文献

ab108659 has not yet been referenced specifically in any publications.

客户评价及客户问答

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ab108659 does not detect TSH in naked mole-rat plasma

Inconclusive Excellent 5/5 (Ease of Use)
Abreviews
This kit was easy to use however, it did not detect TSH in naked mole-rat plasma. The standards worked perfectly, but my experimental samples failed.
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提交于 Jul 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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