人Thrombopoietin ELISA试剂盒(ab100656)

概述

  • 产品名称人Thrombopoietin ELISA试剂盒
    参阅全部 Thrombopoietin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    24.69 pg/ml - 6000 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.82 84% - 105%
    Serum 92.21 78% - 101%
    Plasma 93.44 80% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Thrombopoietin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombopoietin in serum (Human Thrombopoietin concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 相关性Thrombopoietin is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/cMPL, the product of myeloproliferative leukemia virus oncogene. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
  • 细胞定位Secreted
  • 别名
    • C mpl ligand
    • Megakaryocyte colony stimulating factor
    • Megakaryocyte growth and development factor
    • Megakaryocyte stimulating factor
    • MGC163194
    • MGDF
    • MKCSF
    • ML
    • MPL ligand
    • MPLLG
    • Myeloproliferative leukemia virus oncogene ligand
    • OTTHUMP00000210504
    • THPO
    • TPO
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Thrombopoietin ELISA Kit 图像

  • Representative standard curve using ab100656

  • Representative standard curve using ab100656

实验方案

Human Thrombopoietin ELISA Kit (ab100656)参考文献

ab100656 has not yet been referenced specifically in any publications.

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Die beiden monoklonalen Antikörper in diesem Kit sind gegen die Sequenz von ASXX-YY gerichtet, allerdings wurde das Epitop nicht weiter bestimmt. Trotzdem ist es sehr wahrscheinlich, dass das Kit mit Ihre...

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The developer of the kit has sent the following reply addressing your questions about the Thrombopoietin Human ELISA Kit. 1. The plasma samples are collected with the anti-coagulant ACD (acid citrate dextrose) instead of heparin or EDTA. Will that b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"