人Thrombopoietin ELISA试剂盒

(ab100656)

概述

  • 产品名称

    人Thrombopoietin ELISA试剂盒


    参阅全部 Thrombopoietin 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 25 pg/ml
  • 范围
    24.69 pg/ml - 6000 pg/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 97.82 84% - 105%
    Plasma 93.44 80% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Thrombopoietin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thrombopoietin in plasma and cell culture supernatants. Human Thrombopoietin concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect the endogenous Human Thrombopoietin in normal serum with ab100656, only in serum spiked with Human Thrombopoietin.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100656

  • Representative standard curve using ab100656

实验方案

文献

ab100656 has not yet been referenced specifically in any publications.

客户评价及客户问答

Vielen Dank für Ihren Anruf.

Die beiden monoklonalen Antikörper in diesem Kit sind gegen die Sequenz von ASXX-YY gerichtet, allerdings wurde das Epitop nicht weiter bestimmt. Trotzdem ist es sehr wahrscheinlich, dass das Kit mit Ihre...

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The developer of the kit has sent the following reply addressing your questions about the Thrombopoietin Human ELISA Kit. 1. The plasma samples are collected with the anti-coagulant ACD (acid citrate dextrose) instead of heparin or EDTA. Will that b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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