人Thrombomodulin ELISA试剂盒(CD141) (ab46508)

概述

  • 产品名称人Thrombomodulin ELISA试剂盒(CD141)
    参阅全部 Thrombomodulin 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Serum 6 3.9%
    批次间
    样品 n Mean SD CV%
    Serum 6 9.8%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 0.31 ng/ml
  • 范围
    0.625 ng/ml - 20 ng/ml
  • 复检

    109 %

  • 检测时间
    1h 45m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Thrombomodulin (CD141) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombomodulin (CD141) in serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for Thrombomodulin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Thrombomodulin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Thrombomodulin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Thrombomodulin present in the samples.

    This kit will recognize both endogenous and recombinant Human Thrombomodulin.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
  • 组织特异性Endothelial cells are unique in synthesizing thrombomodulin.
  • 疾病相关Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • 序列相似性Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.
  • 翻译后修饰N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • AHUS 6
    • AHUS6
    • BDCA 3
    • BDCA3
    • CD 141
    • CD141
    • CD141 antigen
    • Fetomodulin
    • Thbd
    • THPH12
    • THRM
    • Thrombomodulin
    • TM
    • TRBM_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab46508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Thrombomodulin ELISA Kit (CD141) 图像

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Thrombomodulin measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 2-16 fold.

实验方案

Human Thrombomodulin ELISA Kit (CD141) (ab46508)参考文献

This product has been referenced in:
  • Langdown J  et al. A hereditary bleeding disorder resulting from a premature stop codon in thrombomodulin (p.Cys537Stop). Blood 124:1951-6 (2014). Sandwich ELISA ; Human . Read more (PubMed: 25049278) »
  • Poursafa P  et al. The relationship of air pollution and surrogate markers of endothelial dysfunction in a population-based sample of children. BMC Public Health 11:115 (2011). Read more (PubMed: 21332998) »

See all 3 Publications for this product

Product Wall

Simple & easy human thrombomodulin ELISA

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Followed the data sheet in detail to measure thrombomodulin in human serum.
Well made product and easy to understand.
Would recommend.
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Abcam user community

Verified customer

提交于 Jun 25 2014

Thanks for your enquiry.

My colleague is out of office and he has asked me to look after her customers during her absence.


The whole Thrombomodulin kit is stable if refrigerate (4°C) until at least for one year. However, w...

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Thank you very much for your understanding. The things are more clearer now. Lab has confirmed that the number K100624 (typos K10624) is indeed stands for standard vial.

They have also confirmed that the standard conc. should be between 11-16...

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Thank you for your inquiry.


A panel of 40 human sera and 40 plasma samples were tested for CD141 in a recent batch. See results below

Sample Matrix Number of samples evaluated Range (ng/ml) Mean (ng/ml) Standard deviation (ng...

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Thank you for your telephone enquiry earlier this afternoon. I can confirm that the concentration of the streptavidin - HRP in the Human Thrombomodulin ELISA Kit is 1.0mg/ml. I hope this is helpful. If you have any further questions, please do n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"