概述

  • 产品名称
    人TGF beta 2 ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.14 84% - 105%
    Serum 94.74 82% - 104%
    Plasma 95.53 83% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TGF beta 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 2 in serum, plasma and cell culture supernatants

    This assay employs an antibody specific for Human TGF beta 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab100648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100648

  • Representative standard curve using ab100648

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Thank you for contacting Abcam. Please find the requested Certificate of Compliances attached. If there is anything else that I can help you with, please let me kn

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册