概述

  • 产品名称人TGF beta 2 ELISA试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 15 pg/ml
  • 范围
    8.23 pg/ml - 6000 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 96.14 84% - 105%
    Serum 94.74 82% - 104%
    Plasma 95.53 83% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TGF beta 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 2 in serum, plasma and cell culture supernatants

    This assay employs an antibody specific for Human TGF beta 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
  • 疾病相关Note=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
    Defects in TGFB2 are the cause of Loeys-Dietz syndrome 4 (LDS4) [MIM:614816]. An aortic aneurysm syndrome with widespread systemic involvement. LDS4 is characterized by arterial tortuosity, aortic dissection, intracranial aneurysm and subarachnoid hemorrhage, hypertelorism, bifid uvula, pectus deformity, bicuspid aortic valve, arachnodactyly, scoliosis, foot deformities, dural ectasia, joint hyperflexibility, and thin skin with easy bruising and striae.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • BSC 1 cell growth inhibitor
    • BSC-1 cell growth inhibitor
    • Cetermin
    • G-TSF
    • Glioblastoma derived T cell suppressor factor
    • Glioblastoma-derived T-cell suppressor factor
    • Polyergin
    • TGF beta2
    • TGF-beta-2
    • TGFB2
    • TGFB2_HUMAN
    • Transforming growth factor beta-2
    • Transforming growth factor, beta 2
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human TGF beta 2 ELISA Kit 图像

  • Representative standard curve using ab100648

  • Representative standard curve using ab100648

实验方案

Human TGF beta 2 ELISA Kit (ab100648)参考文献

This product has been referenced in:

See 1 Publication for this product

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