人TGF beta 1 ELISA试剂盒(ab108912)

概述

  • 产品名称人TGF beta 1 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 3.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.6%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 0.03 ng/ml
  • 范围
    0.031 ng/ml - 2 ng/ml
  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TGF beta 1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TGF beta 1 concentrations in cell culture supernatants.

    A TGF beta 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TGF beta 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TGF beta 1 captured in plate. This assay recognizes both natural and recombinant Human TGF beta 1.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 组织特异性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 疾病相关Camurati-Engelmann disease
  • 序列相似性Belongs to the TGF-beta family.
  • 结构域The 'straitjacket' and 'arm' domains encircle the growth factor monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. Activation of TGF-beta1 requires the binding of integrin alpha-V to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-beta binding proteins. The sheer physical force unfastens the straitjacket and releases the active growth factor dimer.
  • 翻译后修饰Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • LAP
    • Latency-associated peptide
    • TGF-beta-1
    • tgfb1
    • TGFB1_HUMAN
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab108912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human TGF beta 1 ELISA Kit 图像

  • Representative Standard Curve using ab108912.

实验方案

Human TGF beta 1 ELISA Kit (ab108912)参考文献

ab108912 has not yet been referenced specifically in any publications.

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