人TGF beta 1 ELISA试剂盒(ab108912)
Key features and details
- Sensitivity: 31 pg/ml
- Range: 0.063 ng/ml - 4 ng/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人TGF beta 1 ELISA试剂盒
参阅全部 TGF beta 1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 60 6.5% 批次间 样品 n Mean SD CV% Overall 60 10.9% -
样品类型
Cell culture supernatant -
检测类型
Sandwich (quantitative) -
灵敏度
= 31 pg/ml -
范围
0.063 ng/ml - 4 ng/ml -
回收率
108 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s TGF beta 1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TGF beta 1 concentrations in cell culture supernatants.
A TGF beta 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TGF beta 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TGF beta 1 captured in plate. This assay recognizes both natural and recombinant Human TGF beta 1.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 30X Biotinylated Human TGF beta 1 Antibody 1 x 180µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml TGF beta 1 Microplate (12 x 8 well strips) 1 unit TGF beta 1 Standard 1 vial -
研究领域
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功能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
组织特异性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
疾病相关
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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别名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
数据库链接
- Entrez Gene: 7040 Human
- Omim: 190180 Human
- SwissProt: P01137 Human
- Unigene: 645227 Human
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab108912 被引用在 4 文献中.
- Lee CH et al. Isoliquiritigenin Inhibits Gastric Cancer Stemness, Modulates Tumor Microenvironment, and Suppresses Tumor Growth through Glucose-Regulated Protein 78 Downregulation. Biomedicines 10:N/A (2022). PubMed: 35740372
- Zhao Q et al. PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. Mol Cell 77:95-107.e5 (2020). PubMed: 31628042
- Li W et al. LncRNA POU3F3 promotes cancer cell migration and invasion in nasopharyngeal carcinoma by up-regulating TGF-ß1. Biosci Rep 39:N/A (2019). PubMed: 30602452
- Zhang H et al. lncRNA MIR4435-2HG promotes cancer cell migration and invasion in prostate carcinoma by upregulating TGF-ß1. Oncol Lett 18:4016-4021 (2019). PubMed: 31516603