概述

  • 产品名称
    人TGF alpha ELISA试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 3 pg/ml
  • 范围
    1.37 pg/ml - 1000 pg/ml
  • 复检

    93 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 94.49 84% - 105%
    Serum 92.34 82% - 104%
    Plasma 93.46 83% - 103%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TGF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF alpha in serum, plasma, cell culture supernatants

    This assay employs an antibody specific for Human TGF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TGF alpha 2 vials
    Recombinant Human TGF alpha Standard 2 vials
    Stop Solution 1 x 8ml
    TGF alpha Microplate (12 x 8 wells) 1 unit
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    Transforming growth factor alpha (TGF alpha) is an acid- and heat-stable originally discovered in rodents and humans. TGF alpha protien is 33% homologous to epidermal growth factor (EGF). TGF alpha binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF alpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells . TGF alpha bioactivity has also been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
  • 别名
    • EGF like TGF
    • ETGF
    • OTTHUMP00000203300
    • OTTHUMP00000203301
    • OTTHUMP00000203345
    • OTTHUMP00000203352
    • Protransforming growth factor alpha
    • RATTGFAA
    • TFGA
    • TGF A
    • TGF type 1
    • TGFA
    • Transforming growth factor alpha
    • Transforming growth factor alpha precursor
    • Wa1
    • Waved 1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100646

  • Representative standard curve using ab100646

实验方案

文献

This product has been referenced in:
  • Hollmén M  et al. G-CSF regulates macrophage phenotype and associates with poor overall survival in human triple-negative breast cancer. Oncoimmunology 5:e1115177 (2016). Human . Read more (PubMed: 27141367) »
  • Mouradian M  et al. Key roles for GRB2-associated-binding protein 1, phosphatidylinositol-3-kinase, cyclooxygenase 2, prostaglandin E2 and transforming growth factor alpha in linoleic acid-induced upregulation of lung and breast cancer cell growth. Prostaglandins Leukot Essent Fatty Acids 90:105-15 (2014). Read more (PubMed: 24374147) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacell...

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Merci de nous avoir contactés.

Le kit ELISA ab100646 est en effet approprié pour le dosage du TGF-alpha humain contenu dans les supernageants de cultures cellulaires, urine, sérum et plasma.

Pour les anti-Histone ...

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Abreview for TGF alpha Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (serum)
Specification: serum
Amount of Sample: 20 uL µg
Type of Kit Used: ELISA
Positive control: We did not measure positive control except for kit standards.
Did you use the Abcam Kit protocol?: Yes
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提交于 Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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