人testis组织裂解液- total protein (ab30257)

概述

  • 产品名称人testis组织裂解液- total protein
    参阅全部 Testis 细胞裂解液
  • 描述
    人testis总蛋白裂解液
  • 常规说明


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • 存储溶液Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Sodium Deoxycholate, Tergitol-NP40, EDTA, Magnesium chloride, HEPES, Sucrose, Potassium chloride, Protease inhibitor, 0.15M Beta mercaptoethanol, pH 7.9
  • Concentration information loading...
  • 裂解物说明Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab30257 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    Human testis tissue lysate - total protein (ab30257)参考文献

    This product has been referenced in:
    • de Bock L  et al. Sperm-associated antigen 16 is a novel target of the humoral autoimmune response in multiple sclerosis. J Immunol 193:2147-56 (2014). Read more (PubMed: 25086173) »
    • Makovski A  et al. Intronic promoter drives the BORIS-regulated expression of FerT in colon carcinoma cells. J Biol Chem : (2012). WB . Read more (PubMed: 22223638) »

    See all 3 Publications for this product

    Product Wall

    Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the quality of this lysate samples.

    I would like to reassure you that ab30257 is tested and covered by our 6 month guarantee for use in western ...

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    Thank you for your telephone call this morning.

    I can confirm as discussed on the telephone that we recommend to load 20 - 30 ug of protein per lane of a gel

    In this case, to work out the volume supplied in each vial and the amoun...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"