人TECK ELISA试剂盒(Thymus Expressed Chemokine) (ab100645)

概述

  • 产品名称
    人TECK ELISA试剂盒(Thymus Expressed Chemokine)
    参阅全部 TECK 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Cell culture supernatant, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    < 200 pg/ml
  • 范围
    0.206 ng/ml - 150 ng/ml
  • 复检

    100 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 109.5 93% - 132%
    Plasma 123.8 113% - 132%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s TECK Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TECK in plasma and cell culture supernatants. (Human TECK concentration is pretty low in normal plasma, it may not be detected in this assay).


    This assay employs an antibody specific for Human TECK coated on a 96-well plate. Standards and samples are pipetted into the wells and TECK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TECK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TECK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    We have not been able to detect endogenous Human TECK in normal serum with ab100645, only in serum spiked with Human TECK.

  • 说明

    Optimization may be requried with urine samples.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    1,000X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human TECK 2 vials
    Recombinant Human TECK Standard 2 vials
    Stop Solution 1 x 25ml
    TECK Microplate (12 x 8 wells) 1 unit
    TMB Substrate Reagent 1 x 12ml
  • 研究领域
  • 相关性
    This gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. TECK displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. TECK binds to chemokine receptor CCR9. Two alternatively spliced variants encoding two different isoforms have been identified.
  • 别名
    • C C motif chemokine 25
    • CCL 25 MGC125074
    • CCL25
    • Chemokine (C C motif) ligand 25
    • Chemokine TECK
    • Ck beta 15
    • Ckb 15
    • Ckb15
    • MGC150327
    • SCY A25
    • SCYA 25
    • SCYA25
    • Small inducible cytokine A25
    • Small inducible cytokine A25 isoform 1 precursor
    • Small inducible cytokine A25 isoform 2 precursor
    • Small inducible cytokine subfamily A (Cys Cys) member 25
    • Small inducible cytokine subfamily A Cys Cys member 25
    • Small inducible cytokine subfamily A, member 25
    • TECKvar
    • Thymus expressed chemokine
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative standard curve using ab100645

  • Representative standard curve using ab100645

实验方案

文献

ab100645 has not yet been referenced specifically in any publications.

客户评价及客户问答

The recommended starting dilution for testing normal serum/plasma with the kit is 2-fold. However, please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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