人Survivin ELISA试剂盒(ab119607)

概述

  • 产品名称人Survivin ELISA试剂盒
    参阅全部 Survivin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    62.5 pg/ml - 4000 pg/ml
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Survivin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Survivin in cell culture supernatants, serum and plasma (heparin, EDTA).


    A Survivin specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for Survivin is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Survivin amount of sample captured in plate.


    For higher reproducibility in only 90 minutes, we recommend our SimpleStep ELISA® range made with recombinant monoclonal antibodies. Try Human Survivin ELISA Kit (ab183361).

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • 组织特异性Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • 序列相似性Belongs to the IAP family.
    Contains 1 BIR repeat.
  • 发展阶段Expression is cell cycle-dependent and peaks at mitosis.
  • 结构域The BIR repeat is necessary and sufficient for HBXIP binding.
  • 翻译后修饰Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • 细胞定位Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt
  • 别名
    • API4
    • Apoptosis inhibitor 4
    • Apoptosis inhibitor survivin
    • Baculoviral IAP repeat containing 5
    • Baculoviral IAP repeat-containing protein 5
    • BIRC5
    • BIRC5_HUMAN
    • EPR 1
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab119607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Survivin ELISA Kit 图像

  • Representative standard curve using ab119607

实验方案

Human Survivin ELISA Kit (ab119607)参考文献

ab119607 has not yet been referenced specifically in any publications.

Product Wall

Vielen Dank für Ihre Anfrage.

Frau Burkhardt ist nun in Ihrem wohlverdienten Urlaub, daher werde ich mich um Ihren Fall kümmern.

Der Schritt, welcher entfällt, ist der folgende:

"7. Preparation "A...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

Read More

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"