人Superoxide Dismutase 1 ELISA试剂盒(ab119520)

概述

  • 产品名称
    人Superoxide Dismutase 1 ELISA试剂盒
    参阅全部 Superoxide Dismutase 1 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.1%
    批次间
    样品 n Mean SD CV%
    Overall 5.8%
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    0.04 ng/ml
  • 范围
    0.08 ng/ml - 5 ng/ml
  • 复检

    98 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 98 89% - 108%

  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Human Superoxide Dismutase 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.


    Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

  • 说明

    ab119520 Human Superoxide Dismutase 1 ELISA kit has been shown to detect both SOD1 and SOD3.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Microplate

性能

应用

Our Abpromise guarantee covers the use of ab119520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Representative Standard Curve using ab119520.

实验方案

文献

This product has been referenced in:
  • Stradling JR  et al. Biomarkers of oxidative stress following continuous positive airway pressure withdrawal: data from two randomised trials. Eur Respir J 46:1065-71 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26022961) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with sampl...

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Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: http://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: http...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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