人stomach组织裂解液(adenocarcinoma) - insoluble fraction (male, 53 years) (differentiated) (ab44337)

概述

  • 产品名称人stomach组织裂解液(adenocarcinoma) - insoluble fraction (male, 53 years) (differentiated)
    参阅全部 Stomach 细胞裂解液
  • 常规说明

    Tissue: Stomach Location: Antrum Diagnosis: Adenocarcinoma, moderately differentiated Stage: IIIA Grade: n/a Sex: Male Age: 53 years Gross findings: Ulcerative lesion located at the lesser curvature, ill demarcated. Tumor measures 5cm in diameter. Cut section is firm and white. Paragastric lymph nodes: 6. Microscopic findings: Tissue sections show proliferation of glandular cells. Mitotic figures are not evident. The epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm. Cytoplasm contains much mucin. Nuclear chromatin is coarse with prominent nuclei. The neoplastic cells form groups and lines and invade the smooth muscle propria and serosa. The stroma is infiltrated by small numbers of lymphocytes, plasma cells, eosinopils and neutrophils. Blood vessel invasions are not revealed. Lymph nodes show metastases. Insoluble protein fraction.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44337: Extraction 1: soluble protein fraction ab44335 Human Stomach Tumor Tissue lysate ab44334 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44337 Human Stomach Tumor Tissue lysate ab44336 Human Stomach Normal Tissue lysate (matched)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 纯化说明Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.

    Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 53 years) (differentiated) (ab44337)参考文献

    ab44337 has not yet been referenced specifically in any publications.

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