人 SCF ELISA试剂盒(Kit Ligand) (ab108901)
Key features and details
- Sensitivity: 0.11 ng/ml
- Range: 0.156 ng/ml - 40 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人 SCF ELISA试剂盒(Kit Ligand)
参阅全部 SCF 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 4.8% 批次间 样品 n Mean SD CV% Overall 9.2% -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.11 ng/ml -
范围
0.156 ng/ml - 40 ng/ml -
回收率
95 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Stem Cell Factor (Kit Ligand) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Stem Cell Factor concentrations in Human plasma, serum and cell culture supernatants.
A Stem Cell Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Stem Cell Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Stem Cell Factor captured in plate.
Store kit at 4°C immediately upon receipt, apart from the Streptavidin-Peroxidase Conjugate & Biotinylated Antibody, which should be stored at -20°C.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 20ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Stem Cell Factor Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stem Cell Factor Microplate (12 x 8 well strips) 1 unit Stem Cell Factor Standard 1 vial Stop Solution 1 x 11ml -
研究领域
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功能
Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. -
疾病相关
Hyperpigmentation with or without hypopigmentation, familial progressive
Deafness, congenital, unilateral or asymmetric -
序列相似性
Belongs to the SCF family. -
发展阶段
Acts in the early stages of hematopoiesis. -
翻译后修饰
A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain. -
细胞定位
Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. - Information by UniProt
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别名
- C kit ligand
- C-kit ligand
- Ckit ligand
see all -
数据库链接
- Entrez Gene: 4254 Human
- Omim: 184745 Human
- SwissProt: P21583 Human
- Unigene: 1048 Human
- Unigene: 661108 Human
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab108901 被引用在 3 文献中.
- Kefayat A et al. Diagnostic and prognostic value of stem cell factor plasma level in glioblastoma multiforme patients. Cancer Med 10:5154-5162 (2021). PubMed: 34250760
- Li G et al. Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model. Sci Rep 8:6566 (2018). PubMed: 29700361
- Lupicka M et al. Identification of pluripotent cells in bovine uterus: in situ and in vitro studies. Reproduction 149:317-27 (2015). Sandwich ELISA . PubMed: 25556182