人Siglec 9 ELISA试剂盒(CD329) (ab113339)

概述

  • 产品名称人Siglec 9 ELISA试剂盒(CD329)
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 130 pg/ml
  • 范围
    0.123 ng/ml - 30 ng/ml
  • 复检

    85 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 95.43 88% - 102%
    Serum 82.7 72% - 91%
    Plasma 78.16 70% - 91%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Siglec 9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Siglec 9 in serum, plasma, cell culture supernatants.

    This assay employs an antibody specific for Human Siglec 9 coated on a 96-well plate. Standards and samples are pipetted into the wells and Siglec 9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Siglec 9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Siglec 9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab113339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Siglec 9 ELISA Kit (CD329) 图像

  • Representative Standard Curve using ab113339

  • Representative Standard Curve using ab113339

实验方案

Human Siglec 9 ELISA Kit (CD329) (ab113339)参考文献

ab113339 has not yet been referenced specifically in any publications.

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Unfortunately, we have not performed epitope mapping so we do not know the exact sequence where the antibody pair in the Siglec 9 Human ELISA Kit is binding. However, we do know that they are binding somewhere on ...

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