Anti-Human Serum Albumin抗体[AL-01] (ab7793)

概述

  • 产品名称Anti-Human Serum Albumin抗体[AL-01]
    参阅全部 Human Serum Albumin 一抗
  • 描述
    小鼠单克隆抗体[AL-01] to人Serum Albumin
  • 特异性We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: ELISA, RIA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fraction of proteins containing albumin after ammonium sulphate precipitation and DEAE-chromatography of human serum.

  • 阳性对照
    • IHC-P: Human liver FFPE tissue sections.

性能

应用

Our Abpromise guarantee covers the use of ab7793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • 组织特异性Plasma.
  • 疾病相关Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • 序列相似性Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • 翻译后修饰Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • Albumin antibody
    • Analbuminemia antibody
    • Bisalbuminemia antibody
    • Cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • Dysalbuminemic hyperthyroxinemia antibody
    • Growth inhibiting protein 20 antibody
    • HSA antibody
    • Hyperthyroxinemia dysalbuminemic antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • Serum albumin antibody
    see all

Anti-Human Serum Albumin antibody [AL-01] 图像

  • IHC image of Human Serum Albumin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7793, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Human Serum Albumin antibody [AL-01] (ab7793) at 1/5000 dilution

    Lane 1 : Healthy control plasma at 0.2 µl
    Lane 2 : Patient plasma at 14 µg
    Lane 3 : Patient plasma at 14 µg
    Lane 4 : Patient plasma at 14 µg
    Lane 5 : Patient plasma at 14 µg
    Lane 6 : Patient plasma at 14 µg
    Lane 7 : Patient plasma at 14 µg
    Lane 8 : Patient plasma at 14 µg
    Lane 9 : Patient plasma at 14 µg
    Lane 10 : Patient plasma at 14 µg

    Secondary
    HRP-conjugated Goat Anti-Mouse IgG polyclonal at 1/25000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 69 kDa


    Exposure time : 15 seconds

    This image is courtesy of an Anonymous Abreview

    See Abreview

Anti-Human Serum Albumin antibody [AL-01] (ab7793)参考文献

This product has been referenced in:
  • Basu J  et al. Human immunodeficiency virus (HIV) antigens and RNA in HIV-seronegative women with cervical intraepithelial neoplasia. AIDS Res Hum Retroviruses 25:249-59 (2009). WB ; Human . Read more (PubMed: 19292595) »
  • Angelisová P  et al. The characteristics of monoclonal antibodies against human albumin. Folia Biol (Praha) 32:289-94 (1986). Read more (PubMed: 3506524) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (whole human plasma)
Loading amount 14 µg
Specification whole human plasma
Treatment heated to 95C in 10-fold excess of reducing SDS sample buffer for 10min
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

提交于 Mar 25 2011

Thank you for your enquiry. All four of these antibodies are monoclonals raised to the full protein. Unfortunately the epitope recognized by each antibody is unknown, as they have not yet been epitope-mapped. All of the information we have for e...

Read More

Thank you for your enquiry. The antibody has been tested and characterized for Western blot application. However, the epitope has not been mapped.

This antibody reacts only with human serum albumin (no cross-reactivity found with other species).

Unfortuinately, we currently do not stock a product to meet your requirements.

Yes, this antibody recognises HSA in WB under reducing conditions.

The concentration of Ig is 1mg/1ml. The suggested starting dilution is 1:500, but it will be necessary to optimise this.

Sorry for the mistake on the datasheet. The antibody is a mouse monoclonal. The rabbit reference was the mistake.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"