抗人Serum Albumin抗体(ab8940)

Abreviews (2)Q&A (19)Specific References (24)

概述

  • 产品名称
    抗人Serum Albumin抗体
    参阅全部 Human Serum Albumin 一抗
  • 描述
    羊多克隆抗体to人Serum Albumin
  • 宿主
    Sheep
  • 经测试应用
    适用于: IHC-Fr, Double Immunodiffusion, Immunoelectrophoresis, RID, IHC-P, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • 免疫原

    Human serum albumin purified from Cohn Fraction V.

性能

应用

Our Abpromise guarantee covers the use of ab8940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Double Immunodiffusion Use at an assay dependent concentration. 10µL antiserum vs 5µL serum (1/20 dilution).
Immunoelectrophoresis Use at an assay dependent concentration.

2µL antiserum/cm² gel vs 5µL 1/200 - 1/2000 dilution human serum IEP: 100µL antiserum vs 1µL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily be excluded.
RID Use at an assay dependent concentration.
IHC-P 1/1000. See Abreview.
IHC-FoFr Use at an assay dependent concentration.

靶标

图片

  • Immunohistochemistry (Frozen sections) - Human Serum Albumin antibody (ab8940)
    Immunohistochemistry (Frozen sections) - Human Serum Albumin antibody (ab8940)This image is courtesy of an Abreview submitted by Dr Asha Seth

    ab8940 at 1/1000 staining mouse embryonic tissue sections (E10.5) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with 3ml maleate bufer, 1ml FCS, 1ML 10% blocking powder before incubation with the primary antibody for 16 hours at 37°C. A Cy3® conjugated donkey anti-sheep antibody was used as the secondary.

    The image shows hepatoblasts in the liver bud positive for albumin (red) and also some expressing E-cadherin (green).

    See Abreview

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Human Serum Albumin antibody (ab8940)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Human Serum Albumin antibody (ab8940)Image from Cotugno G et al., PLoS One. 2012;7(3):e33286. Epub 2012 Mar 13. Fig 3.; doi:10.1371/journal.pone.0033286; March 13, 2012, PLoS ONE 7(3): e33286.
    Immunohistochemical analysis of AAV2/8 injected rat liver tissue, staining Human Serum Albumin (red) with ab8940.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100, and blocked with 5% normal rabbit serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight. An AlexaFluor®594-conjugated anti-sheep IgG (1/1000) was used as the secondary antibody.

文献

This product has been referenced in:
  • Saito Y  et al. Induction of differentiation of intrahepatic cholangiocarcinoma cells to functional hepatocytes using an organoid culture system. Sci Rep 8:2821 (2018). Read more (PubMed: 29434290) »
  • Tang J  et al. Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human. Biomed Rep 6:627-632 (2017). Read more (PubMed: 28584633) »

See all 24 Publications for this product

发表研究结果有使用 ab8940？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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21 reviews or Q&A

10
Votes: Highest First

Hello,

You say below that the discount applies to primary antibodies. We have now tracked the problem to the secondary (Cy5 labelled anti-sheep antibodies). Does the discount apply to these?

Regards

Thank you for your message.

I am sorry to hear you have continued to experience difficulties, butampleased you have found the source of the problem. Was the Cy5 anti sheep antibody from Abcam? Do you have the product code, and I will be plea...

Read More

I may want to test ab10241 in IHC-P.

Thank you very much for your interest in ab10241.

To our knowledge, ab10241 has not been tested in IHC-P. Therefore, I can offer a discount off a future purchase if you buy ab10241 now, test it in IHC-P and submit feedback to us in the form ...

Read More

Muchas gracias por su respuesta. Cree que el mismo anticuerpo primario podría servir en un western? Gracias otra vez

Lamentablemente no puedo asegurarte que el anticuerpo vaya a funcionar en WB. Nosotros no lo hemos testado en el laboratorio, por lo que no tenemos evidencia de que vaya a reconocer la proteína en esta aplicación.

Siento no pode...

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Finalmente como abes he comprado ab8940 anti-human serum albumin

y ab6743 anti sheep, la primera prueba no ha sido demasiado exitosa,

por lo que quería preguntar, si teneis algun protocolo de refencia.

Muchas gra...

Read More

Gracias por tu respuesta.

No tenemos un protocolo concreto para el uso de este anticuerpo mediante immunohistoquímica, sino protocolos generales que pueden servir de guía para los distintos ensayos.

Te adjunto el link ...

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Hello,

I would like to inquire about Anti-Human Serum Albumin antibody (ab8940). I am reviewing the details about this antibody and I am not sure about purity of this IgG. Could you tell me that this product doesnt contain any proteinexcepti...

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Thank you for your enquiry.

I can confirm that this particular product, ab8940,is sourced externally. Using zone electrophoresis under standard conditions, the originator has shown that only sheep IgG is present, however if more sensitive te...

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Hello,

Thanks for your reply.

We will pay for the new antibodies with the 50% discount.

How do we get this discount? Is there an identifier that need to be included in the order?

Also, are we able to obtain a...

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Thank you for your message.

I can confirm you can quote the discount code previously provided to you when you place the order:

As requested, as a gesture of goodwill, I have set up a 50% discount which is valid for the purchase of...

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Hello,

About a year ago we had a problem with antibodies purchased from your company. The problem was never resolved and your request for your supplier to investigate was never acted on by them. We have encountered this problem again and we ...

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Thank you for your message.

I am sincerely sorry we did not manage to resolve this issue for you. The originator did not have any further information to provide on this occasion. As previously discussed, this antibody has not been tested in ...

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Muchas gracias por tus comentarios.
Como sistema de recuperación antigénica utilizo normalmente citrato sódico y microondas.
Me comentas lo de usar suero de mismo secundario, pero en este caso no seri sheep?

Mu...

Read More

Gracias por tu respuesta.
Te confirmo que ab53435 solo se ha testado en secciones congeladas. No tenemos, pues, ninguna evidencia de que vaya a detectar el epítopo en secciones incluidas en parafina.
Así pues, puedes optar por testarlo en est...

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Si, pero quería utilizarlo en parafina y parece que sólo funciona el de rata en congelado.

Por otro lado, el de humano pero para gran espectro si que funciona en parafina,

estás de acuerdo...? Me asegurais que...

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Gracias por tu respuesta.

Como bien indicas, ab53435 solo se ha testado en secciones congeladas, no tengo constancia de que funcione en parafina. De todas maneras, he contactado con mis compañeros del laboratorio para que me lo confirm...

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¿Sería posible que me enviarais una muestra de prueba de ab8940 para testarla antes de comprarla?
Muchas gracias

Muchas gracias por contactarnos de nuevo.

Lamentablemente, ya que tenemos en el catálogo más de 80.000 productos, nos resulta imposible guardar muestras de prueba de todos ellos para enviar como alícuotas de test.
Read More

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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