- 数据表
- 具体的参考文献 (11)
- 实验方案
概述
- 产品名称Anti-Human Serum Albumin抗体
参阅全部 Human Serum Albumin 一抗 - 描述羊多克隆抗体to人Serum Albumin
- 经测试应用适用于: IHC-Fr, Double Immunodiffusion, Immunoelectrophoresis, RID, IHC-P, IHC-FoFrmore details
- 种属反应性与反应: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
- 免疫原
Human serum albumin purified from Cohn Fraction V.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
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Concentration information loading... - 纯度IgG fraction
- 克隆多克隆
- 同种型IgG
- 轻链类型unknown
- 研究领域
应用
Our Abpromise guarantee covers the use of ab8940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| Double Immunodiffusion | Use at an assay dependent concentration. 10µL antiserum vs 5µL serum (1/20 dilution). | |
| Immunoelectrophoresis | Use at an assay dependent concentration. 2µL antiserum/cm² gel vs 5µL 1/200 - 1/2000 dilution human serum IEP: 100µL antiserum vs 1µL serum The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbentassays and Western blot has not been assessed but use in such assays should not necessarily be excluded. |
|
| RID | Use at an assay dependent concentration. | |
| IHC-P | 1/1000. See Abreview. | |
| IHC-FoFr | Use at an assay dependent concentration. |
靶标
- 功能Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
- 组织特异性Plasma.
- 疾病相关Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
- 序列相似性Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. - 翻译后修饰Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. - 细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 448843 Cat
- Entrez Gene: 396197 Chicken
- Entrez Gene: 280717 Cow
- Entrez Gene: 100860821 Goat
- Entrez Gene: 100379232 Guinea pig
- Entrez Gene: 100034206 Horse
- Entrez Gene: 213 Human
- Entrez Gene: 11657 Mouse
see all -
别名
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Anti-Human Serum Albumin antibody 图像
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Immunohistochemistry (Frozen sections) - Human Serum Albumin antibody (ab8940)This image is courtesy of an Abreview submitted by Dr Asha Sethab8940 at 1/1000 staining mouse embryonic tissue sections (E10.5) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with 3ml maleate bufer, 1ml FCS, 1ML 10% blocking powder before incubation with the primary antibody for 16 hours at 37°C. A Cy3® conjugated donkey anti-sheep antibody was used as the secondary.
The image shows hepatoblasts in the liver bud positive for albumin (red) and also some expressing E-cadherin (green).
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Human Serum Albumin antibody (ab8940)Image from Cotugno G et al., PLoS One. 2012;7(3):e33286. Epub 2012 Mar 13. Fig 3.; doi:10.1371/journal.pone.0033286; March 13, 2012, PLoS ONE 7(3): e33286.Immunohistochemical analysis of AAV2/8 injected rat liver tissue, staining Human Serum Albumin (red) with ab8940.
Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100, and blocked with 5% normal rabbit serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) overnight. An AlexaFluor®594-conjugated anti-sheep IgG (1/1000) was used as the secondary antibody.
Anti-Human Serum Albumin antibody (ab8940)参考文献
This product has been referenced in:
- Solà-Adell C et al. Calcium Dobesilate Prevents Neurodegeneration and Vascular Leakage in Experimental Diabetes. Curr Eye Res N/A:1-14 (2017). Read more (PubMed: 28574750) »
- Gao Y et al. The Chinese Herbal Medicine Formula mKG Suppresses Pulmonary Fibrosis of Mice Induced by Bleomycin. Int J Mol Sci 17:238 (2016). Mouse . Read more (PubMed: 26891294) »
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