人Semaphorin 3c肽(ab39299)

概述

描述

  • 性质Synthetic

技术指标

Our Abpromise guarantee covers the use of ab39299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-Semaphorin 3c antibody (ab39300)

  • 形式Liquid
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

常规信息

  • 别名
    • Sema E
    • SEMA3C
    • semaE
    • Semahorin3C
    • Semaphorin E
    • SemaphorinE
    see all
  • 相关性The Semaphorins constitute a large family of secreted and membrane-tethered cell signaling molecules. They have functions in neural development, immunology, cardiac growth, vascular development, lung morphogenesis and axial bone patterning. Semaphorins are defined by the presence of a conserved Sema domain at the N-terminus. These proteins can be classified into eight classes depending on their structure and species origin. Classes 3 through 7 are found in vertebrates; class 3 are secreted, class 7 are GPI-anchored and class 4 through 6 are transmembrane proteins. Two distinct transmembrane receptor families have been identified as Semaphorin receptors. Neuropilins provide binding specificity for the class 3 Semaphorins, whereas Plexins, which also contain a Sema domain serve as signaling partners for class 3 Semaphorins and as functional receptors for transmembrane Semaphorins. Semaphorin 3c inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is expressed maximally between days E10-12 with moderate levels from day E13 until birth.
  • 细胞定位Secreted

Human Semaphorin 3c peptide (ab39299)参考文献

ab39299 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application ELISA
We used this peptide as a positive control for an anti-netrin 1 peptide antibody in an ELISA assay. The peptide worked very well. We also found that the peptide as a potent chemorepellant in our cell system. We plan to use this peptide as a positive control for our Western Blots as well.
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Verified customer

提交于 Sep 03 2014

Thank you for your patience.

I spoke to my colleague Jill who is in charge to source new proteins, and even she agrees that we should have this protein in our catalog, I am afraid this will take quite a while ( more than two month at least). ...

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Thank you for contacting us again.

I think the problem with this peptide is that it will probably only be detected by the polyclonal. I cannot tell you if the monoclonal antibody can bind this peptide as the epitope wasn't mapped.

...

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