人Retinol binding protein 4 ELISA试剂盒(RBP4) (ab108897)

概述

  • 产品名称人Retinol binding protein 4 ELISA试剂盒(RBP4)
    参阅全部 Retinol binding Protein 4 试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.8%
    批次间
    样品 n Mean SD CV%
    Overall 9%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    = 6 ng/ml
  • 范围
    6.25 ng/ml - 400 ng/ml
  • 复检

    99.5 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Retinol binding protein 4 (RBP4) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Retinol binding protein 4 concentrations in plasma, serum, saliva, urine, milk, cerebrospinal fluid and cell culture supernatants.


    A Retinol binding protein 4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Retinol binding protein 4specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Retinol binding protein 4 captured in plate.


    For higher sensitivity in only 90 minutes, we recommend Human Retinol Binding Protein 4 ELISA Kit (ab196264) from our SimpleStep ELISA® range.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
  • 疾病相关Retinal dystrophy, iris coloboma, and comedogenic acne syndrome
  • 序列相似性Belongs to the calycin superfamily. Lipocalin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Plasma retinol binding protein
    • Plasma retinol-binding protein
    • Plasma retinol-binding protein(1-176)
    • PRBP
    • PRO2222
    • RBP
    • RBP4
    • RDCCAS
    • RET4_HUMAN
    • Retinol binding protein 4
    • Retinol binding protein 4 interstitial
    • Retinol binding protein 4 plasma
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution.

Human Retinol binding protein 4 ELISA Kit (RBP4) 图像

  • Representative Standard Curve Using ab108897.

实验方案

Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)参考文献

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Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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