概述

  • 产品名称人Resistin ELISA试剂盒
    参阅全部 Resistin 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    < 2 pg/ml
  • 范围
    1.4 pg/ml - 400 pg/ml
  • 复检

    95 %

    特定样本复检结果
    样品类型 平均% 范围
    Cell culture supernatant 98.42 89% - 108%
    Serum 93.59 78% - 102%
    Plasma 94.31 79% - 103%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Resistin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Resistin in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 说明

    Optimization may be required with urine samples.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
  • 组织特异性Expressed only in fatty tissues.
  • 序列相似性Belongs to the resistin/FIZZ family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Adipose tissue specific secretory factor
    • Adipose tissue-specific secretory factor
    • ADSF
    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein
    • C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 1
    • C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein
    • Cysteine rich secreted protein A12 alpha like 2
    • Cysteine rich secreted protein FIZZ3
    • Cysteine-rich secreted protein A12-alpha-like 2
    • Cysteine-rich secreted protein FIZZ3
    • FIZZ 3
    • FIZZ3
    • Found in inflammatory zone 3
    • HXCP 1
    • HXCP1
    • MGC126603
    • MGC126609
    • PRO1199
    • Resistin
    • Resistin delta2
    • RETN
    • RETN 1
    • RETN_HUMAN
    • RETN1
    • UNQ407
    • XCP 1
    • XCP1
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab100634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human Resistin ELISA Kit 图像

  • Representative Standard Curve using ab100634.

  • Representative Standard Curve using ab100634.

实验方案

Human Resistin ELISA Kit (ab100634)参考文献

ab100634 has not yet been referenced specifically in any publications.

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