人Resistin ELISA试剂盒(ab100634)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.4 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Resistin ELISA试剂盒
参阅全部 Resistin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
1.4 pg/ml - 400 pg/ml -
回收率
95 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 98.42 89% - 108% Serum 93.59 78% - 102% Plasma 94.31 79% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Resistin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Resistin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 100X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Resistin 2 vials Recombinant Human Resistin Standard (lyophilized) 2 vials Resistin Microplate (12 x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
组织特异性
Expressed only in fatty tissues. -
序列相似性
Belongs to the resistin/FIZZ family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
数据库链接
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100634 被引用在 2 文献中.
- Zhao L et al. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus. Ther Clin Risk Manag 13:739-750 (2017). PubMed: 28721055
- Quan H et al. A crossover study of the combination therapy of metformin and exenatide or biphasic insulin aspart 30 in overweight or obese patients newly diagnosed with type 2 diabetes mellitus. Exp Ther Med 14:3279-3287 (2017). PubMed: 28912879