概述

  • 产品名称人PSGL1 ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 8 3.2%
    批次间
    样品 n Mean SD CV%
    Overall 8 6.6%
  • 测试
    1 x 96 test
  • 样品类型
    Cell culture supernatant, Serum
  • 检测类型Sandwich (quantitative)
  • 灵敏度
    1 U/ml
  • 范围
    1.6 U/ml - 50 U/ml
  • 复检

    94 %

    特定样本复检结果
    样品类型 平均% 范围
    Serum 94 83% - 104%

  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s PSGL1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PSGL1 concentrations in cell culture supernatant and serum.

    Human PSGL1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and then incubated at room temperature. After washing, a Biotin-conjugated anti-Human PSGL1 detection antibody is added then incubated at room temperature. Following washing Streptavidin-HRP conjugate is added to each well, incubated at room temperature then again washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human PSGL1 captured on the plate.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Microplate

性能

  • 功能A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.
  • 组织特异性Expressed on neutrophils, monocytes and most lymphocytes.
  • 翻译后修饰Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
    Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
  • 细胞定位Membrane.
  • Information by UniProt
  • 别名
    • CD162
    • CD162 antigen
    • CLA
    • Cutaneous lymphocyte associated associated antigen
    • P-selectin glycoprotein ligand 1
    • PSGL 1
    • PSGL-1
    • Selectin P ligand
    • SELPL_HUMAN
    • SELPLG
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab119555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

Human PSGL1 ELISA Kit 图像

  • Representative Standard Curve using ab119555.

实验方案

Human PSGL1 ELISA Kit (ab119555)参考文献

ab119555 has not yet been referenced specifically in any publications.

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