概述

  • 产品名称人Protein S ELISA试剂盒
  • 检测方法Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5.1%
    批次间
    样品 n Mean SD CV%
    Overall 7.4%
  • 测试
    1 x 96 well plate
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型Competitive
  • 灵敏度
    0.25 µg/ml
  • 范围
    0.25 µg/ml - 8 µg/ml
  • 复检

    98.5 %

  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Abcam’s Protein S Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human protein S in plasma, serum and cell culture supernatants.

    A Protein S specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein S is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein S captured in plate.

  • 经测试应用适用于: Competitive ELISAmore details
  • 平台Microplate

性能

  • 功能Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
  • 组织特异性Plasma.
  • 疾病相关Defects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity.
  • 序列相似性Contains 4 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 laminin G-like domains.
  • 翻译后修饰The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • 细胞定位Secreted.
  • Information by UniProt
  • 别名
    • Preproprotein S
    • Propiece of latent protein S
    • PROS
    • PROS 1
    • PROS_HUMAN
    • proS1
    • Protein S alpha
    • Protein Sa
    • PS 21
    • PS 22
    • PS 23
    • PS 24
    • PS 25
    • PS 26
    • PS21
    • PS22
    • PS23
    • PS24
    • PS25
    • PS26
    • PSA
    • THPH5
    • THPH6
    • Vitamin K dependent protein S
    • Vitamin K-dependent plasma protein S
    • Vitamin K-dependent protein S
    see all
  • 数据库链接

应用

Our Abpromise guarantee covers the use of ab125969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

Human Protein S ELISA Kit 图像

  • Representative Standard Curve using ab125969

实验方案

Human Protein S ELISA Kit (ab125969)参考文献

ab125969 has not yet been referenced specifically in any publications.

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